Constitutional Chips 4.0

Constitutional Chips 4.0 (PerkinElmer®)

Description

Comparative Genomic Hybridization (microarray-CGH) is a technology based on detection of genomic imbalance and chromosomal aberrations (abnormalities of a few hundred kilobases up to several megabases). This technology allows quantitative analysis of genome abnormalities with a a resolution of 50 to 100 times higher than with constitutional karyotyping.
The Constitutionnal Chip 4.0 is an innovation in cytogenetics for continuous development of molecular diagnostics and research. This chip has applications in many fields and study areas, such as neonatal and prenatal diagnostics, oncology and stem cells.

Features

Some Constitutionnal Chip 4.0 features:

  • No amplification of DNA samples is required prior to hybridization.
  • The chip offers a high resolution view of the entire genome, without pre-selection of deficient genes, unlike FISH (Fluorescent In-Situ Hybridization)
  • It provides a comprehensive coverage of clinically significant loci.
  • Cell culture is not required, unlike conventionnal karyotyping techniques.
  • This technology allows obtaining of results in less than 2 days.
Reference Designation
4060-0010 Constitutional Chip 4.0 (10 slides)
4040-0020 Labelling Reagents (for 10 Microarrays)
4050-0010 Hybridization Reagents (10 Microarrays)

Download the flyer Constitutional Chip 4.0 (PerkinElmer®)

Technical Features

The Constitutional Chip 4.0 offers:

  • More than 1200 clones covering regions responsable for known microdeletions and microduplications (Average resolution = 450 kb).
  • 600 clones are dedicated to X chromosome (Average resolution = 250 kb).
  • 900 clones are dedicated to subtelomeric regions.
  • Pericentromeric regions are also covered to facilitate prenatal diagnostics.
  • Constitutionnal Chip 4.0 has an average global resolution of 650 kb, corresponding to approximately 25% of the human genome.
  • Microarray on Standard Glass Slide, adapted to all systems using this type of support.

Hybridization & Labelling Reagents Kits content:

 
1. Hybridization Reagents:

• 1 vial (495 µl) of Hybridization Buffer 1:
Hybridization Buffer 1 contains human Cot-1 DNA,sheared salmon testis DNA, and NaCl.
• 1 vial (330 µl) of Hybridization Buffer 2:
Hybridization Buffer 2 contains SSC (Saline Sodium Citrate), formamide and Dextran.
Warning: Formamide is toxic. Use with caution.
• 1 vial (1200 µL) of aCGH Water:
aCGH Water is RNAase/DNAase-free water.

 

2. Labelling reagents: 

• 1 vial (140 µl) of labelling buffer:
This buffer contains dATP, dCTP, dGTP, dTTP on base of Tris-HCl buffer.
• 1 vial (425 µl) of Random primers:
This buffer contains random primer oligonucletides on base of Tris-HCl buffer.
• 1 vial (55 µl) of Klenow:
This buffer is a DNA polymerase.
• 1 vial (45 µl) of Cyanine 3:
This vial contains dCTP-Cy3 on base of Tris-HCl buffer.
Warning: keep the vial protected from light.
• 1 vial (45 µl) of Cyanine 5:
This vial contains dCTP-Cy5 on base of Tris-HCl buffer.
Warning: keep the vial protected from light.

All Hybridization and Labelling reagents must be stored at -30/-16°c. Expiry date is stated on the vial label.

3.Wash Buffer Pack:

• Prewash Buffer : SSC 2X, SDS 0,1% 40 ml for 10 slides
• Wash Buffer 1: SSC 1X, SDS 0,1% 400 ml for 10 slides
• Wash Buffer 2: SSC 0,1X, SDS 0,1% 200 ml for 10 slides
• Wash Buffer 3: SSC 0,1X 400 ml for 10 slides
• Rinse Solution: Isopropanol 200 ml for 10 slides

All solutions are stored at room temperature.