Wash Buffers

Wash buffer for cDNAs and long oligonucleotides

Description

wash buffers_abc.jpg This Wash Buffers are composition-optimized to provide the greatest hybridization stringency possible, while preserving intense signals and ensuring low background. Wash Buffers A, B and C are used for cDNA and long oligonucleotide microarrays.
Reference Designation
WBA High salt and detergent wash buffer for cDNAs and long oligonucleotides (>25-mers), low stringency wash buffer, 1 1iter of 10X concentrate
WBB Low salt and detergent wash buffer for cDNAs and long oligonucleotides (>25-mers), high stringency wash buffer, 1 1iter of 10X concentrate
WBC Very low salt wash buffer for cDNAs and long oligonucleotides (>25-mers), rinse buffer, 1 1iter of 10X concentrate

Main Characteristics

  • High stringency without reducing signal intensities
  • Superior formulation reduces background
  • All buffers purified by 0.2 µm sterile filtration
  • Subjected to lot-by-lot quality control (QC) and quality assurance (QA)
  • Provided in 1 liter bottles as 10X concentrates
  • Each liter of 10X buffer will wash up to 500 microarrays


Protocol

The Wash buffers must be first diluted to get a 1X concentration.

 Following hybridization steps :
1- Wash for 5 min at room temperature in 500 ml 1X Wash Buffer A..
2- Wash for 5 min at room temperature in 500 ml 1X Wash Buffer B.
3- Dunk for 1 sec at room temperature in 50 ml 1X Wash Buffer C.
4- Spin dry and scan. For spin drying BioTray advises to use the High Speed Microarray Centrifuge.

For improving speed and quality of results BioTray advises to do each wash with the High Throughput Microarray Wash Station.

Wash buffer for short oligonucleotides (<25 mers) microarray experiments

Description

wash buffers_123.jpg This Wash Buffers are composition-optimized to provide the greatest hybridization stringency possible, while preserving intense signals and ensuring low background. Wash Buffers 1, 2 and 3 are designed for short oligonucleotide (<25-mer) microarrays.
Reference Designation
WB1  High salt and detergent wash buffer for short oligonucleotides (<25-mers), low stringency wash, 1 1iter of 10X concentrate
WB2  Low salt and detergent wash buffer for short oligonucleotides (<25-mers), high stringency wash buffer, 1 1iter of 10X concentrate
WB3  Very low salt wash buffer for short oligonucleotides (<25-mers), rinse buffer, 1 1iter of 10X concentrate

Main Characteristics

  • High stringency without reducing signal intensities
  • Superior formulation reduces background
  • All buffers purified by 0.2 µm sterile filtration
  • Subjected to lot-by-lot quality control (QC) and quality assurance (QA)
  • Provided in 1 liter bottles as 10X concentrates
  • Each liter of 10X buffer will wash up to 500 microarrays

Protocol

The Wash buffers must be first diluted to get a 1X concentration.

Following hybridization steps :
1- Wash for 5 min at room temperature in 500 ml 1X Wash Buffer 1.
2- Wash for 5 min at room temperature in 500 ml 1X Wash Buffer 2.
3- Dunk for 1 sec at room temperature in 50 ml 1X Wash Buffer 3.
4- Spin dry and scan. For spin drying BioTray advises to use the High Speed Microarray Centrifuge.