Ozone Free Box

Ozone Free Box™
Ozone Free Environment

Ozone Free Box

360° Presentation


The Ozone Free Box™ is a comprehensive and effective solution to prevent the fluorescent dyes which are used for samples labelling in microarray applications (Cyanine, Alex Fluor etc...) from being oxidized and degraded by ozone.

The fluorescent dyes degradation because of ozone has been experimentally demonstrated after exposure to ozone levels as low as 5 to 10 ppb (0.005 to 0.010 ppm) for period as short as 10 to 30 seconds. This attack by ozone happens during sample and microarray preparations, hybridization, washes, drying and scanning stages.

Ozone effects on microarray are:
  • Signal intensities reduction due to fluorescent dyes oxidization by ozone
  • Erroneous fluorescent dyes ratio in dual-color hybridization experiments and false analysis and interpretation as a consequence
  • Huge loss of money due to spoiled slides, wasted reagents, wasted samples and wrong data.

BioTray®'s Ozone Free Box™ is a powerful solution which totally destroy ozone in order to have an ozone free environment during samples preparation, hybridization, washes, drying and scanning stages.
BioTray®'s Ozone Free Box™ consists on a workspace in which the user can work and put his instruments, coupled together with a catalytic converter unit which recycles the air and destroys ozone and a highly sensitive ozone sensor for real time monitoring of ozone level.
The workspace was design to be perfectly adapted to the tasks of microarray users and the equipment they work with. 
The catalytic converter unit uses a special filter technology combined with an effective air recycling system that ensure a total destruction of ozone inside the workspace : as a consequence the ozone levels is under 1 ppb when the workspace's doors are closed and the ozone level is maintained and stabilized below 5 ppb when workspace's doors are open.
A centrifuge fan with adjustable speed adapts the recycling intensity to the state of the doors (open or closed) as well as the level of external ozone pollution, consequently allowing the users to work within the Ozone Free Box™ workspace with an ozone level  always maintained under 5 ppb, whatever the external conditions might be. 
A high precision ozone sensor monitors the ozone level inside the Ozone Free Box™ workspace in real time to control and guarantee that the users are working being  effectively protected from ozone.

BioTray®'s Ozone Free Box™ currently is the unique affordable solution against ozone that  has these technical performances in terms of ozone destruction, ozone level control and stabilization when the workspace doors are open.
4 standards are provided in order to cover the various applications and equipment (type of scanner) of customers.

 Reference  Description of Ozone Free Box™ product
 OFB_80x150  Dimensions (D x H x L): 80 x 80 x 171 cm
 Workspace: (D x L): 80 x 150 cm
 OFB_100x200  Dimensions (D x H x L): 80 x 100 x 221 cm
 Workspace: (D x L): 80 x 200 cm

Download the flyer Ozone Free Box


Dimensions 4 standards are provided according to user's application and equipment. Please contact us for further evaluation.
Box & Catalytic Converter Unit
Anodized Aluminium profiles
Transparent & blue PMMA
Doors Anodized Aluminium profiles with PMMA plates and handles
Vertical opening: 2 jacks for each door
Side door mounted on hinges
Number of doors: 2 for th OFB_100x200
Catalyst Filters
Aluminium filters with Manganase Dioxide (MnO2). Design by BioTray®
Dimensions: 125 x 125 mm. Thickness: 15 mm
High Precision Sensor Mounted on a sensor head holder inside the box
Connected into a monitor with digital display with a cable and a RJ45 connector
Resolution: +/- 0.001 ppm (1ppb)
Calibrated range: 0.000 – 0.050 ppm
Response time: < 70s
Operating temperatures: -5° to 50°C
Power supply : 12 VDC, 800 mA
Centrifuge Fan
1650 rpm – 105 Watt – 220 V
With speed variator
Digital Display
Positionned on the front of the catalytic converter unit
Real time monitoring of ozone levels
Programmable alarms
Power Supply
220V - 105 Watts
ON/OFF switch on the front and on the side of the catalytic converter unit

 Catalyst filters:

Fig.1: Catalyst Filters

The filters in aluminium are coated with Manganase Dioxide (MnO2). Manganase Dioxide is a material with black color which is used as a catalyst. The specific design of our filters significantly improves the destruction rate of ozone with only 1.5 m/s as a linear air flow velocity.
Fig.1: Filtre Catalyseur

The experiments below were run in parallele, using the very same sample. Slide 1 was hybridized and scanned at the ambient (fig.2), slide 2 was hybridized under zero ozone atmosphere (fig.3).

Fig.2: Hybridized chips at the amnient (with ozone).
Fig.3: Hybridized chips under  zero-ozone atmosphere. 

High precision sensor:

The Ozone free Box™ has a high precision sensor with very tight resolution (1 ppb) in order to precisely measure ozone concentration even at low levels. The sensor head is plugged onto a holder fixed inside the workspace and it is connected into a digital display, which is positionned in the front of the catalyst converter unit, thanks to a cable and a RJ 45 connector.
The sensor has a very short response time (<70 s) allowing real time monitoring of ozone levels. An alarm can be configured by the user in order to alert him when the ozone level rises above a defined value (5 ppb is advised as a relevant value in microarray applications).
As an option, sensing features can be upgraded in order to measure temperature and relative humidity as well.

Fig.4: High precision sensor Fig.5: Control panel with digital display


Catalyst Filters:
The Ozone Free Box™ solutions need only two catalyst filters.The filters must be replaced every 6 months in the case of a very intensive use or once a year in the case of periodic use.The filters are made with aluminium and a Manganase Dioxide (MnO2) coating. Furthermore a specific design explains the high ozone destruction rate : it improves the ozone destruction effisciency and reduces the time needed to purify the air inside the Ozone Free Box™ from ozone.
Fig.1: Catalyst filter

Reference Designation
CF Catalyst Filter ;  Dimensions: 12,5 x 12,5 cm. Thickness: 15 mm

Ozone Sensor Head:

The Ozone Sensor Head is carefully calibrated in a controlled environment. The calibration environment has a certified Zero-Ozone atmosphere with tightly regulated Temperature (20°C ± 2°C) and Relative humidity (50% ± 5%) conditions.
The "Zero-Ozone" value is saved in the microprocessor memory of the control panel with digital display.
The ozone sensor head consists in a membrane filter, a pump as an active sampling mechanism, and a semiconductor.
Fig.2: Sonde du capteur d'ozone

The air is sucked in the sensor head by the pump and goes through it. The electrical resistance of the semiconductor changes when ozone is detected by its sensing surface. Then sensor driver algorithms convert this electrical resistance variations into an accurate ozone level displayed on the control panel.
Some Volatile Organic Compounds (VOCs) present in the air are contaminants that may settle on the sensing surface inside the sensor head. As a consequence, the Zero-Ozone value drifts and then, the displayed ozone level is erroneous.
Volatile organic compounds (VOCs) refers to organic chemical compounds (composed with carbon and hydrogen) which are found in their gaseous state in the air ; VOCs are numerous, varied, and ubiquitous ; VOCs include both man-made (factory emissions, organic solvents vaporisation) and naturally occurring (plants emissions) chemical compounds ; the anthropogenic VOCs are regulated, especially for indoors where concentrations can be highest.

An auto calibration process is integrated into the ozone sensor and it can be run in routine using the control panel. This operation is automated, simple and effective. The semiconductor is warmed up in order to vaporize the contaminants and remove them from the sensing surface. This auto calibration operation eliminates contaminants problems and zero ozone value drift : then the control panel displays an accurate and correct ozone level.

The ozone sensor head must be replace every 6 months in case of intensive daily use or every one year in case of periodic use.

Reference Description
 BTZ Ozone Sensor Head
Calibration Range/ 0 to 0.050 ppm (0 to 50 ppb)
Response Time: <70s
Operating Temperature: -5°C to 50 °C

AlphaLite Wipers:

Fig.3: AlphaLite® Wipers

Fig.3: AlphaLite wipers are lightweight knitted wipers constructed of 100% continuous-filament polyester. They are cleanroom laundered to provide an ultraclean, adsorbent wiper that is ideal for critical cleanroom and biomedical applications ; they are really suitable for wiping and spill control with their low particle counts, highly absorbent properties, low extractable levels and solvent compatibility.

BioTray® advises to use AlphaLite wipers together with CipperMop cleanroom mops in order to clean the Ozone Free Box™. These wipers when fastened on ClipperMop head mop provide an quick, effective and effortless solution to clean the outside and inside parts of the Ozone Free Box™.

Important Note : the walls of the Ozone Free Box™ are made in a material that must not be exposed to solvents. If you wish to use other products than pure water, please refer to the user guide before or contact us.

Reference Description
TX 1008  AlphaLite wipers
Size: 9"x9" (23 x 23 cm)
Packaging: 150 wipers per bags.
2 inner bags of 75 wipers

ClipperMop™ Cleanroom mops:

Fig.4: Cleanroom mop with telescopic handle for easy cleaning Fig.5: ClipperMop™ mop head with AlphaLite® wiper

Fig.4: ClipperMop™ cleanroom mops are the ideal tool to quick and easy cleaning of flat vertical and horizontal surfaces and hard-to-reach areas (walls, ceiling ...) in cleanroom, pharmaceutical, biomedical and medical environments.
ClipperMop™ consists in a telescopic handle that can extended up to 134cm and in a mop head. The mop head utilizes non-abrasive polyethylene clips to securely fasten one or multiple wipers to the head ; it has a flexible polyester pad for better wiping efficiency even on hard-to-reach surfaces and a replaceable polyester foam pad ensuring that wipers conform to uneven surfaces.

Fig.5: Lateral view of the mop head with its flexible polyester pad, the polyester foam pad and a AlphaLite® wiper fasten on it.

BioTray® advises to use ClipperMop™ cleanroom mops together with AlphaLite® wipers in order to clean the Ozone Free Box™. The telescopic handle and the flexible mop head give an easy access to each wall of the anti ozone cabinet with no need to enter inside the box. The lightweight knitted wiper constructed of 100% continuous-filament polyester allows to slide and clean the surfaces with no effort so that the operator will not tire himself out cleaning the Ozone Free Box™.

Important Note : the walls of the Ozone Free Box are made in a material that must not be exposed to solvents. If you wish to use other products than pure water, please refer to the user guide before or contact us.

Reference Description
TX 7102 ClipperMop™ cleanroom mop
Packaging : 1 case with 1 Cleanroom mop with 7" x 4" (18 cm x 10 cm) head assembly and telescoping handle inside.


Ozone Free Box™ for Agilent scanner


Ozone Free Box™ for Innopsys scanner

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Ozone Free Box™ for various scanners

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